HaRIC_B characterization. A. SHR incidence means for inbred lines carrying HaRIC_B haplotypes 1, 2 and 3 (H1, H2, H3). Error bars refer to standard error. B. PCR amplification of HaRIC_B using genomic (gDNA) and cDNA of inbred lines carrying HaRIC_B haplotypes 1, 2 and 3, respectively. C. Gene structure, polymorphisms found in haplotypes 2 or 3 compared to haplotype 1, and splicing patterns of HaRIC_B for the different haplotypes found in the AMP. D. Expression of HaRIC_B haplotypes 1 (H1) and 3 (H3) in different plant organs. RT-PCR for HaRIC_B (HaR) and Actin (A) for leaf (leaf), stem (st), root (root), florets at R3 (fR3), receptacle at R3 (rR3), florets at R5.2 (fR5.2), receptacles at R5.2 (rR5.2), florets at R6 (fR6), receptacle at R6 (rR6) and dry seeds (seed).
Fusari et al. BMC Plant Biology 2012 12:93 doi:10.1186/1471-2229-12-93