Table 5

Sequence Based Polymorphic (SBP) markers generated for thePSS1region
Name 1Primer 2Enzyme AmpliconSize (bp)
SBP_22.95 F: GGAGGTTCCGTTACTC TTACTG R: CCACCGGAA GACGACGACTCTTC RsaI 309
SBP_22.98 F: CGACGTCACACTCTCC GTTA R: CCGATGATGGA GAAGGAAAA TaqI 230
SBP_23.06 F: AAATTGGGGACACCA ACAAA R: GGTCCTCCTG GGAGAAAGAT Tsp509I 180
SBP_23.09 F: TCGAATGATCCTTTCC TTTCA R: GCTTTTGCGA AAATGGGATA TaqI 235
SBP_23.46 F: GACCAAATGTCTCTGA GATGTTC R: ACCCAAGG CGGTGTTGGCGAAAG TaqI 520

1Primers: F, forward primer; R, reverse primer. 2Restriction endonucleases used for individual CAPS markers are shown

Sumit et al.

Sumit et al. BMC Plant Biology 2012 12:87   doi:10.1186/1471-2229-12-87

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