Additional file 2.

A: Autoflourescene ofpss1mutant leaf. Detached leaves of 21-day old seedlings of the pss1 mutant were mock inoculated with sterile water and stained with aniline blue and observed under ultraviolet epiflourescence 6 hours post inoculation. The image was taken at 50X magnification. The experiment was repeated three times with similar results. B: Thepss1mutant is a host for soybean oomycete pathogen,P. sojae. Detached leaves of pss1mutant were inoculated with P. sojae zoospores (105 spores/ml.) and stained with trypan blue dye 7 days post inoculation (dpi). Formation of sexual female reproductive structures, oogonia (oo) and asexual reproductive structures, sporangia (sp) indicate that the pathogen is able to complete its life cycle on the host pss1 mutant leaves, thus signifying a complete breakdown of Arabidopsis nonhost resistance in this mutant. Numbers indicate the approximate size of the reproductive structures, which is in close agreement with the average size of the reproductive structures of the Phytophthora genus [45].

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Sumit et al. BMC Plant Biology 2012 12:87   doi:10.1186/1471-2229-12-87