Analysis of polypeptide composition of wild-type and chy1chy2lut2lut5 thylakoid membrane. (A) Pigment-protein complexes isolated by sucrose density gradient fractionation (Figure 5B) were analyzed by Tris-tricine (left panel) of Tris-glycine (right panel) SDS-PAGE. Main protein components of each fraction are indicated. MW, molecular weight marker. (B) Results of the immuno-titration of thylakoid proteins. Immunoblot analyses were performed with antibodies directed against individual gene products. Data of PSII subunits were normalized to the PsbB content, that of Lhca to the PsaB content, and expressed as a percentage of the corresponding wild-type content. Significantly different values in protein amount than the corresponding wild-type, according to Student's t test (P < 0.05), are marked (*).
Fiore et al. BMC Plant Biology 2012 12:50 doi:10.1186/1471-2229-12-50