Open Access Research article

A quadruple mutant of Arabidopsis reveals a β-carotene hydroxylation activity for LUT1/CYP97C1 and a regulatory role of xanthophylls on determination of the PSI/PSII ratio

Alessia Fiore1, Luca Dall'Osto2, Stefano Cazzaniga2, Gianfranco Diretto1, Giovanni Giuliano1 and Roberto Bassi234*

Author Affiliations

1 Italian National Agency for New Technologies, Energy and Sustainable Development (ENEA), Casaccia Research Center, Via Anguillarese 301, 00123 Rome, Italy

2 Dipartimento di Biotecnologie, Università di Verona, Strada Le Grazie 15, 37134 Verona, Italy

3 ICG-3: Phytosphäre Forschungszentrum Jülich, 52425 Jülich, Germany

4 Dipartimento di Biotecnologie, Università di Verona, Strada Le Grazie 15, 37134 Verona, Italy

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BMC Plant Biology 2012, 12:50  doi:10.1186/1471-2229-12-50

Published: 18 April 2012

Additional files

Additional file 1:

Figure S1. Genomic structure of the different mutants utilized. Figure S2. PCR confirmation of the different mutants. Figure S3. Isolation and characterization of the pigment-protein complexes from wild-type and chy1chy2lut2lut5 thylakoid membrane. Figure S4. Distribution of the PSI core (A), PSII core (B) and LHCII (B) amount per fresh weight vs. the relative content of xanthophylls on thylakoids. Table S1. Sequences of oligonucleotides used for RT-PCR measurement of transcripts. Table S2. LC-DAD-MS analysis of wild-type and mutant Arabidopsis leaves.

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