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Open Access Highly Accessed Research article

Generation and analysis of blueberry transcriptome sequences from leaves, developing fruit, and flower buds from cold acclimation through deacclimation

Lisa J Rowland1*, Nadim Alkharouf2, Omar Darwish2, Elizabeth L Ogden1, James J Polashock3, Nahla V Bassil4 and Dorrie Main5

Author Affiliations

1 Genetic Improvement of Fruits and Vegetables Laboratory, USDA-ARS, BARC-West, 10300 Baltimore Ave., Beltsville, MD 20705, USA

2 Department of Computer and Information Sciences, Towson University, 8000 York Road, Towson, MD 21252, USA

3 Genetic Improvement of Fruits and Vegetables Laboratory, USDA-ARS, Blueberry and Cranberry Research Center, 125A Lake Oswego Road, Chatsworth, NJ 08019, USA

4 National Clonal Germplasm Repository, USDA-ARS, 33447 Peoria Road, Corvallis, OR 97333-2521, USA

5 Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA, USA

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BMC Plant Biology 2012, 12:46  doi:10.1186/1471-2229-12-46

Published: 2 April 2012

Additional files

Additional file 1:

Most highly abundant sequences from each of the libraries along with their number of reads/frequency of reads and predicted expression patterns across the bud time points and the fruit development stages. Approximately 30 most highly abundant sequences from each of the libraries along with their number of reads/frequency of reads and predicted expression patterns across the bud time points (0, 397, 789, and 1333 chill hours) and fruit development stages (green, white, pink, and blue/ripe).

Format: XLSX Size: 103KB Download file

Open Data

Additional file 2:

Summary of amplification and polymorphism results from screening 100 EST-SSRs on parents of tetraploid and diploid mapping populations of blueberry. Result summary of amplification and polymorphism of 100 EST-SSRs in parents of a tetraploid mapping population 'Draper' and 'Jewel' and two progeny individuals, BB 05-61-1 and BB 05-61-2; and in a diploid testcross mapping population between #10 [Fla4B (V. darrowii) × W85-20 (V. corymbosum)] and W85-23. Fla4B and W85-20 were also included. Prefix VCB is in the SSR name and precedes the EST name and stands for Vaccinium corymbosum 'Bluecrop', the source of the ESTs. Polymorphic alleles are highlighted. Y indicates in ORF while N indicates absence from ORF.

Format: DOCX Size: 61KB Download file

Open Data