Differential effects of environment on potato phenylpropanoid and carotenoid expression
1 Irrigated Agricultural Research and Extension Center, Washington State University, Prosser, WA 99350, USA
2 USDA-Agricultural Research Service, Washington State University, 24106 N. Bunn Rd, Prosser, WA 99350, USA
3 The Department of Plant, Soil, and Entomological Sciences, University of Idaho, P.O. Box 442339, Moscow, ID 84844, USA
4 USDA- Agricultural Research Service, Subarctic Agricultural Research Unit, P.O. Box 757200, Fairbanks, AK, USA
BMC Plant Biology 2012, 12:39 doi:10.1186/1471-2229-12-39Published: 20 March 2012
Additional file 1:
Table of phenolic compounds measured in tubers by LCMS. Retention time (Rt) and MS data of analyzed compounds is shown.
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Additional file 2:
Relative expression of five genes involved in primary metabolism. DAHP, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; PGK, phosphoglycerate kinase; AMY, alpha-amylase; SUSY, sucrose synthase; SSY, soluble starch synthase. The data represents the means ± SE of three biological replicates. Locations with same letter are not significantly different (p < 0.05).
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Additional file 3:
Carotenoid profiles in Alaska (A), Texas (B) and Florida (C) samples. Major peaks are 1. neoxanthin, 2. violaxanthin, 3. antheraxanthin, 4. lutein, 5. zeaxanthin, 6. β-apo-caroten-8-ol internal standard.
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Additional file 4:
Correlation analysis of carotenoid transcript and metabolite levels in tubers. Pearson correlation coefficients were calculated and used to generate a heatmap in which positive correlations are shown in red and negative correlations in blue. Phen, total phenolics; Anth, total anthocyanins; Cartn, total carotenoids; FRAP, antioxidant capacity. Additional abbreviations are listed in Figure 8 and Additional file 1.
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Additional file 5:
Table of HPLC data of tuber carotenoids.
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Additional file 6:
Sequence information for primers.
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