Open Access Research article

Combinatorial analysis of lupulin gland transcription factors from R2R3Myb, bHLH and WDR families indicates a complex regulation of chs_H1 genes essential for prenylflavonoid biosynthesis in hop (Humulus Lupulus L.)

Jaroslav Matoušek13*, Tomáš Kocábek1, Josef Patzak2, Zoltán Füssy13, Jitka Procházková1 and Arne Heyerick4

Author Affiliations

1 Biology Centre ASCR v.v.i, Institute of Plant Molecular Biology, Branišovská 31, 370 05 České Budějovice, Czech Republic

2 Hop Research Institute, Co. Ltd, Kadaňská 2525, 438 46 Žatec, Czech Republic

3 Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic

4 Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium

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BMC Plant Biology 2012, 12:27  doi:10.1186/1471-2229-12-27

Published: 20 February 2012

Additional files

Additional file 1:

List of R2R3Myb TFs included in the phylogenetic tree presented in Figure 1 and alignment of amino acid sequences within R2R3 domain.

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Additional file 2:

Comparative analysis of the 3-D protein structures of the N-terminal/R2/R3 regions of HlMyb2, l-HlMyb3 and s-HlMyb3. The theoretical structures were calculated and portrayed against the template 1h88C.pdb using the SWISS-MODEL Workspace. The alignments of the 3-D structures were performed using the Swiss-PdbViewer v3.7b2 (see Methods). Structures are presented as single ribbons, positions of R2 and R3 repeats are shown on alignment s-HlMyb3/l-HlMyb3; the positions of essential residues forming the bHLH-binding site of HlMyb2 are shown on the structure alignment for s-HlMyb3/HlMyb2. The positions of hydrophobic amino acids substituting the bHLH-binding residues in s-HlMyb3 are between brackets. The positions of the largest structural deviations are indicated on the structures by filled and hollow arrows. N and C are N- and C- terminus, respectively.

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Additional file 3:

List of bHLH TFs included in the phylogenetic tree presented in Figure 2A and alignment of amino acid sequences within bHLH domain.

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Additional file 4:

List of WDR TFs included in the phylogenetic tree presented in Figure 3Aand alignment of amino acid sequences.

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Additional file 5:

Relative levels of anthocyanin pigments in petunia leaves (%) infiltrated with different hop TFs or AtPAP1 compared to anthocyanins in petunia dark blue corollas performed by HPLC analysis.

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Additional file 6:

Oligonucleotide primers used in this study. Table of oligonucleotide primers used in this study, arranged according the purpose for which they were designated.

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Additional file 7:

List of Agrobacterium tumefaciens strains and vectors. Table of A. tumefaciens strains and vectors used in the analysis of lupulin gland transcription factors from R2R3Myb, bHLH and WDR families.

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Additional file 8:

Histochemical and quantitative analysis of GUS activity driven by modified variants of Pchs_H1 by hop TFs (supplement to Table 1).

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