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Open Access Highly Accessed Research article

Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat

Xiaofei Zhang12, Hui Jin1, Yan Zhang1, Dongcheng Liu2, Genying Li3, Xianchun Xia1, Zhonghu He14* and Aimin Zhang2*

Author Affiliations

1 Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China

2 State Key Laboratory of Plant Cell and Chromosome Engineering, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 1 West Beichen Road, Beijing, 100101, China

3 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China

4 International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China

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BMC Plant Biology 2012, 12:243  doi:10.1186/1471-2229-12-243

Published: 22 December 2012

Additional files

Additional file 1: Figure S1:

Quantitative gel densitometry measurements of B-group proteins in Glu-B3 NILs as determined using NIL ImageJ software program. Data points were normalized with respect to the Aroona value. Figure S2: Sequence alignments of i-type proteins identified in Aroona NILs. Figure S3: Sequence alignments of the B3-530 protein and its allelic variants identified in Aroona NILs. Figure S4: Sequence alignments of the B3-544/593/601/607 proteins identified in Aroona NILs. Figure S5: Sequence alignments of the B3-621/624 and B3-688 proteins identified in Aroona NILs. Figure S6: The differential display of B3-688 spots among Aroona-Glu-B3c, B3d and B3h. Spot volume values are expressed in percentages (%vol) of the total proteome.

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Additional file 2: Table S1:

F values of one way ANOVA of wheat quality properties by locations and genotypes. Table S2: Comparison of pan bread quality properties among Aroona NILs. Table S3: Comparison of dough properties among Aroona NILs. Table S4: Comparison of glutenin macro-polymer properties among Aroona NILs. Table S5: Composition of glutenin alleles in Aroona and its NILs.

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