Expression analysis of RR12, 13, 14, 16, 19 by RT-PCR. RNAs isolated from roots (R), stems (S), petioles (P) and leaves (L) under control (c) or stressed (s) conditions, were reverse transcribed and used as template for PCR amplification. PCR reactions were performed using B-type RRs specific primers under optimal conditions for each primer set (A: 30 cycles, B: 40 cycles). Expression profile of a housekeeping gene, Clathrin (CLA), was realized with 25 cycles of PCR amplification.
Bertheau et al. BMC Plant Biology 2012 12:241 doi:10.1186/1471-2229-12-241