Morphological analysis of pollen from p5cs1 homozygous mutants. In support of a proline requirement for pollen development, morphologic analysis of pollen from p5cs1 homozygous mutants revealed the presence of degenerated pollen grains (A-D), unstained with Alexander's stain (D) and lacking visible nuclei with DAPI staining (C, and bright-field control in B). Up to three nuclei are visible in pollen from wild type control (F, and bright-field control in E). Bars= 50 μm (A), 25 μm (B-D).
Mattioli et al. BMC Plant Biology 2012 12:236 doi:10.1186/1471-2229-12-236