DAPI analysis of pollen from wild type and p5cs1 p5cs2/P5CS2. (A) DAPI staining of mature pollen from p5cs1 p5cs2/P5CS2. The small misshaped pollen of the mutant pollen population appears highly degenerated and depleted of nucleus. In contrast the larger and wild-type looking pollen grains display up to three nuclei, as in normal wild type pollen. (B) Bright-field image of the same picture showing large and small mutant pollen grains. Bar = 25 μm.
Mattioli et al. BMC Plant Biology 2012 12:236 doi:10.1186/1471-2229-12-236