Alexander's stain of pollen from wild type and p5cs1 p5cs2/P5CS2 plants. Alexander's staining of pollen and anthers from wild type (A,C) compared to p5cs1 p5cs2/P5CS2 mutant (B,D) reveals that a fraction of the mutant pollen population, looking small and misshaped, is not viable, as does not assume Alexander's stain. In contrast the remaining fraction of the pollen population appears as red as, and indistinguishable from wild type pollen. A detail of pollen grains from p5cs1 p5cs2/P5CS2 mutant is shown in the inset at higher magnification. Bars = 50 μm.
Mattioli et al. BMC Plant Biology 2012 12:236 doi:10.1186/1471-2229-12-236