In vitro germination assays of pollen from p5cs1 p5cs2/P5CS2 plants. To assess the viability of pollen from p5cs1 p5cs2/P5CS2 plants, pollen from wild type (A and, at higher magnification, C) and p5cs1 p5cs2/P5CS2 (B and, at higher magnification, D) was incubated in vitro on germination medium and scored for successful germination (pollen tube fully or partially elongated). In panel (E) the percentage of germinated versus total pollen is shown for wild type (left column) and mutant pollen (right column). In (F) the percentage of germination over total pollen is given for either large (middle column) or small (rightmost column) mutant pollen, compared to wild type (leftmost column). Bars = 100 μm (A, B) and 25 μm (C,D). Values in (E) and (F) represent the means of four independent experiments ± SE.
Mattioli et al. BMC Plant Biology 2012 12:236 doi:10.1186/1471-2229-12-236