Additional file 4.

Root hair deformations and nodule primordia in MtN5-silenced and control roots. A-B. Representative micrographs of root hair curling in M. truncatula roots inoculated with S. meliloti. A. Transgenic adventitious root bearing the MtN5hp gene construct. B. Transgenic adventitious root bearing an empty T-DNA (Control sample). C-D. Representative micrographs of root nodule primordia in M. truncatula roots inoculated with S. meliloti carrying the pXLGD4 plasmid. The presence of rhizobia within the primordia was highlighted through the staining for the β-galactosidase activity. C. Nodule primordium generated on transgenic adventitious root bearing the MtN5hp gene construct. D. Nodule primordium generated on transgenic adventitious root bearing an empty T-DNA (Control sample).

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Pii et al. BMC Plant Biology 2012 12:233   doi:10.1186/1471-2229-12-233