Additional file 3.
Description of NF4257 insertional mutant and determination of DMI1 expression in the mutated background. A. Schematic drawing of the Tnt1 insertion and representation of the oligonucleotides used for the genetic characterization of the insertional line. B. qRT-PCR analysis of DMI1 expression in M. truncatula R108 wild-type and NF4257 roots inoculated with S. meliloti. The expression data were normalized to an internal actin control. The relative expression ratio was calculated using inoculated wild type roots as calibrator sample. The values reported are means ± SE (n=3). Student’s t test was applied. **, P < 0.01.
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Pii et al. BMC Plant Biology 2012 12:233 doi:10.1186/1471-2229-12-233