Expression level of NIN and MtN5 genes in M. truncatula DMI1 insertional mutant line. The expression level of NIN (A) and MtN5 (B) was assessed in M. truncatula R108 wild-type and DMI1 insertional mutant roots, both mock-inoculated and inoculated with S. meliloti. The data were normalized to an internal actin control. The relative expression ratios were calculated using mock-inoculated roots as calibrator sample. The values reported are means ± SE (n=3). Student’s t test was applied. **, P < 0.01; ***, P < 0.001.
Pii et al. BMC Plant Biology 2012 12:233 doi:10.1186/1471-2229-12-233