Validation of the suppression subtractive hybridization (SSH) results by semi-quantitative RT-PCR. (A) Semi-quantitative RT-PCR analysis of eEF-1α on the same RNA samples from BP034 and GM077 as used in the SHH experiment, i.e., RNA from seeds of 12 day after flowering (Se12), and samples from the same time point for leaves (L12), roots (R12), and stems (St12). (B) Semi-quantitative RT-PCR analysis of UBQ5 on RNA samples as in the SHH experiment (Se12), and for seeds from 3, 6, 18 and 24 day after flowering, and for leaves (L12), roots (R12), and Stems (St12), respectively. (C) Semi-quantitative RT-PCR analysis of cDNA levels of eEF-1α before and after subtractive hybridization.
Zhang et al. BMC Plant Biology 2012 12:230 doi:10.1186/1471-2229-12-230