Analysis of cis(+)-OPDA in P. patens WT, ΔPpAOS1 and ΔPpAOS2.cis(+)-OPDA was extracted from unwounded (control) and wounded (1h after wounding stimulus) moss by employing the methyl-tert-butyl ether technique  and analyzed via LC/MS . Shown are the results from two independent experimental datasets. Data are presented as mean values with standard deviations from two - six biological replicates. Values with significant differences (Students T-Test; P < 0.05) are indicated above the respective column by different letters.
Scholz et al. BMC Plant Biology 2012 12:228 doi:10.1186/1471-2229-12-228