Table 4

Characterization of candidate loci identified through mapping of an “enhanced sensitivity to sulfamethoxazole” (ESS) phenotype
Locus Mutations Identified by Mapping T-DNA Lines for Phenotypic Confirmation
Nucleotide Changea Strand Genome Positionb Amino Acid Change Line(s) Examined Phenotype on 0.5 μM Sulfamethoxazolec
At2g23470 G → A - 10000583 G219E CS26759 Bleached
At2g24590 G → A + 10450926 n/ad SALK_023090, SALK_032699C, SALK_094266 Green
At2g25320 G → A - 10785456 G732R SALK_072877C, SALK_007242C Green
At2g26135 C → T + 11130249 A43V SALK_150146C, SALK_055187 Green
At2g27790 C → T - 11847919 T529I SAIL_1155_B02, SAIL_1155_E08, SALK_062215C Green
Wildtype n/a n/a n/a n/a n/a Green
ESS 3-10 n/a n/a n/a n/a n/a Bleached

aCandidate loci were identified as described in Methods. The nucleotide change is shown in the context of the coding sequence, independent of strand orientation.

bGenome position as defined by the Arabidopsis Information Resource.

cSeedling phenotypes were evaluated after 14 days of growth on media containing 3 μM sulfamethoxazole. “Bleached”/”Green” describe the appearance of the majority of seedlings for each genotype and represent assessments from at least two independent experiments.

n/a = not applicable.

Schreiber et al.

Schreiber et al. BMC Plant Biology 2012 12:226   doi:10.1186/1471-2229-12-226

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