|Characterization of candidate loci identified through mapping of an “enhanced sensitivity to sulfamethoxazole” (ESS) phenotype|
|Locus||Mutations Identified by Mapping||T-DNA Lines for Phenotypic Confirmation|
|Nucleotide Changea||Strand||Genome Positionb||Amino Acid Change||Line(s) Examined||Phenotype on 0.5 μM Sulfamethoxazolec|
|At2g23470||G → A||-||10000583||G219E||CS26759||Bleached|
|At2g24590||G → A||+||10450926||n/ad||SALK_023090, SALK_032699C, SALK_094266||Green|
|At2g25320||G → A||-||10785456||G732R||SALK_072877C, SALK_007242C||Green|
|At2g26135||C → T||+||11130249||A43V||SALK_150146C, SALK_055187||Green|
|At2g27790||C → T||-||11847919||T529I||SAIL_1155_B02, SAIL_1155_E08, SALK_062215C||Green|
aCandidate loci were identified as described in Methods. The nucleotide change is shown in the context of the coding sequence, independent of strand orientation.
bGenome position as defined by the Arabidopsis Information Resource.
cSeedling phenotypes were evaluated after 14 days of growth on media containing 3 μM sulfamethoxazole. “Bleached”/”Green” describe the appearance of the majority of seedlings for each genotype and represent assessments from at least two independent experiments.
n/a = not applicable.
Schreiber et al. BMC Plant Biology 2012 12:226 doi:10.1186/1471-2229-12-226