Open Access Highly Accessed Research article

'Le Rouge et le Noir': A decline in flavone formation correlates with the rare color of black dahlia (Dahlia variabilis hort.) flowers

Jana Thill, Silvija Miosic, Romel Ahmed, Karin Schlangen, Gerlinde Muster, Karl Stich and Heidi Halbwirth*

Author Affiliations

Technische Universität Wien, Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Getreidemarkt 9/1665, Wien, A-1060, Austria

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BMC Plant Biology 2012, 12:225  doi:10.1186/1471-2229-12-225

Published: 23 November 2012

Additional files

Additional file 1:

Proportional amounts of flavones, flavonols and anthocyanins in the white cv. White Alva (1), yellow cvs. Rubens (2) Alva’s Supreme (3), red cvs. Feuerschein (4), Cheerio (5),and black cvs. Arabian Night (6), Auroras Kiss (7), Black Jack (8), Chat Noir (9), Charles de Gaulle (10), Karma Choc (11), Magic Night (12), Meteor (13), Natal (14), Negerkopf (15), Tisa (16), Black Barbara (17). For absolute values refer to Table 1.

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Additional file 2:

Flavone synthase II assay in three black cultivars. Radiochromatogram of TLC on cellulose with solvent system CAW (chloroform/acetic acid/water: 10/9/1) from incubation of [14C]eriodictyol in the presence of microsomal preparations from petals of cv. Black Barabara (left), cv. Chat noir (centre) and cv. Stefanie Hertel (right).

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Additional file 3:

Relative expression of structural genes and transcription factors. Expression was determined in comparison to the housekeeping gene actin in the white cv. White Alva (1), yellow cvs. Rubens (2) Alva’s Supreme (3), red cvs. Feuerschein (4), Cheerio (5), and black cvs. Arabian Night (6), Auroras Kiss (7), Black Jack (8), Charles de Gaulle (9), Chat Noir (10), Karma Choc (11), Magic Night (12), Meteor (13), Mythos (14), Negerkopf (15), Tisa (16), Black Barbara (17).

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Additional file 4:

Relative expression of structural genes and transcription factors. Expression was determined in comparison to the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in the white cv. White Alva (1), yellow cvs. Rubens (2) Alva’s Supreme (3), red cvs. Feuerschein (4), Cheerio (5), and black cvs. Arabian Night (6), Auroras Kiss (7), Black Jack (8), Charles de Gaulle (9), Chat Noir (10), Karma Choc (11), Magic Night (12), Meteor (13), Mythos (14), Negerkopf (15), Tisa (16), Black Barbara (17).

Format: TIFF Size: 340KB Download file

Open Data