Temporal analysis of the effect of hormones abscisic acid (ABA) and indole-3-acetic acid (IAA) with or without application of pathogen Fusarium culmorum on the accumulation of select transcripts in heads of barley cultivar Lux. Transcripts represented are: (a) serpin Z4 (Paz1), (b) subtilisin-chymotrypsin inhibitor (CI-1B), (c) tonoplast aquaporin (TIP3:1), (d) zinc methallothionin-like protein (ZnMT), (e) putative non-specific lipid transfer protein (nsLTP), (f) nine-cis-epoxycarotenoid dioxygenase NCED and (g) a signalling cascade protein (Pi2). Transcripts were previously identified by microarray analysis as being primed by the bacterium to accumulate in response to the pathogen at either 24 or 48 h post-pathogen treatment (a to e) or as being activated by the bacterium only (f and g). Treatments: barley heads were treated with IAA, ABA or Tween20 (control treatment), and 24 h later, with pathogen (P) or Tween20. RNA extracted from treated head tissue at either 4, 12, 24 or 48 h post-hormone or hormone and pathogen treatment was used for real-time RT-PCR analysis. aTranscript accumulation was quantified as 2-(CT target transcript–CTα-tubulin). Treatment codes: C, controls treated with Tween20; P, pathogen (F. culmorum); IAA, indole-3-acetic acid; ABA, abscisic acid; IAA + P; IAA plus pathogen; ABA+P, ABA plus pathogen. Results are based on two biological replicates, each including two techical replicates per treatment. Bars indicate standard error of mean.
Petti et al. BMC Plant Biology 2012 12:224 doi:10.1186/1471-2229-12-224