Additional file 4.

PCR of SBE RNAi 4.9 T1genomic DNA. A PCR was performed using the primers Hairpin Fw and Rev (Figure‚ÄČ1a) to detect presence of the transgene hairpin in the genomic DNA of SBE RNAi 4.9 T1 grains. Primers for GAPDH was used as a positive control. The experiment was conducted in triplicate showing the same result.

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Carciofi et al. BMC Plant Biology 2012 12:223   doi:10.1186/1471-2229-12-223