Identification of LMW-GS of B. distachyon by Western blotting and Southern blotting.a. The gel stained by Coomassie brilliant blue after Western blotting. b. Western blotting analysis of B. distachyon. The sample number of lane 1–6 from left to right was Brachypodium accession Bd4 (PI208216), Bd21, Bd16 (PI239715), Bd13 (PI233228), Bd11 (PI226629) and Bd10 (PI226452), respectively. c. Southern blotting analysis of Bd21. Genomic DNA from Bd21 was digested with BamHI, EcoRI and HindIII restriction enzymes.
Wang et al. BMC Plant Biology 2012 12:221 doi:10.1186/1471-2229-12-221