Separation and characterization of grain proteins from 6 Brachypodium accessions and common wheat varieties Chinese Spring (CS) and Kontrast (Kon) by SDS-PAGE. The sample number of lane 1–6 from left to right was Brachypodium accession Bd4 (PI208216), Bd21, Bd16 (PI239715), Bd13 (PI233228), Bd11 (PI226629) and Bd10 (PI226452), respectively. a. Albumins; b. Globulins; c. Albumins and globulins; d. Gliadins; e. LMW glutenin subunits. Different glutenin groups were indicated by A (HMW-GS), B (B-type LMW-GS), C (C-type LMW-GS) and D (D-type LMW-GS), respectively. Arrows indicate 5 representative LMW-GS bands (4–1, 21–1, 21–2, 13-1and 16–1) selected for further identification by MALDI-TOF-MS.
Wang et al. BMC Plant Biology 2012 12:221 doi:10.1186/1471-2229-12-221