Open Access Research article

Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species

Shunli Wang1, Ke Wang2, Guanxing Chen1, Dongwen Lv1, Xiaofeng Han1, Zitong Yu1, Xiaohui Li1, Xingguo Ye2*, SLK Hsam3, Wujun Ma4*, Rudi Appels4 and Yueming Yan1*

Author Affiliations

1 Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100048, Beijing, China

2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, 100081, Beijing, China

3 Division of Plant Breeding and Applied Genetics, Technical University of Munich, D-85350, Freising-Weihenstephan, Germany

4 State Agriculture Biotechnology Centre, Murdoch University; Western Australian Department of Agriculture and Food, Perth, WA, 6150, Australia

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BMC Plant Biology 2012, 12:221  doi:10.1186/1471-2229-12-221

Published: 21 November 2012

Additional files

Additional file 1:

PCR amplification products on agarose gel of 6B. distachyonaccessions. The samples of lane 1–6 from left to right are Brachypodium accession Bd4 (PI208216), Bd10 (PI226452), Bd11 (PI226629), Bd13 (PI233228), Bd16 (PI239715) and Bd21. M represents 1Kb DNA marker, a. PCR amplification results by primer 1 and 2, b. PCR amplification results by primer 7 and 8.

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Additional file 2:

MALDI mass spectrum of the tryptic peptides of the protein band 4–1 from Bd4 which was markedin Figure1.

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Additional file 3:

MALDI mass spectrum of the tryptic peptides of the protein band 22–1 from Bd21 which was marked in Figure1.

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Additional file 4:

MALDI mass spectrum of the tryptic peptides of the protein band13–1 from Bd13 which was marked in Figure1.

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Additional file 5:

MALDI mass spectrum of the tryptic peptides of the protein band 10–1 from Bd10 which was marked in Figure1.

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Additional file 6:

MALDI mass spectrum of the tryptic peptides of the protein band 21–2 from Bd21 which was marked in Figure1.

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Additional file 7:

Protein identification of 21–2 inB. distachyonby MALDI-TOF-MS.

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