Expression analysis of ZmNAC genes and miR164 in various maize tissues, including roots, leaves, ears, leaf sheaths, stems, and male spikes, as determined by real-time PCR. The expression levels of each NAC gene in leaf was chosen as the calibrator. To perform the miR164 expression analysis, an RNA gel blot containing low-molecular-weight RNA (10 μg low molecular weight RNA per lane) was hybridized with an end-labeled DNA oligonucleotide that was complementary to miR164.
Li et al. BMC Plant Biology 2012 12:220 doi:10.1186/1471-2229-12-220