Multiple copies of POS9 and POS6 regions can replicate P-INO function. Structures of the POS9 (−1187 to −874) and POS6 (−873 to −628) promoter region constructs are shown. The orange box represents a 60 bp minimal promoter element from the CaMV 35S promoter. Ovule specific INO-like GUS activity indicates the number of plants exhibiting the outer integument specific GUS staining pattern shown by the full-length P-INO relative to the total number of transformants evaluated, and percentage calculated from these values. * Results obtained when an 8X elevated concentration of X-gluc substrate was used. ** Results obtained when a 5X elevated concentration of X-gluc substrate was used. The frequencies of patterns of expression for pRJM307 and pRJM293 were previously described , and were experimentally repeated in this assay with similar results shown here.
Simon et al. BMC Plant Biology 2012 12:214 doi:10.1186/1471-2229-12-214