Functional characterization of a tomato COBRA-like gene functioning in fruit development and ripening
- Equal contributors
1 Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, China
2 School of Biotechnology and food Engineering, Hefei University of Technology, Hefei, 230009, China
3 US Department of Agriculture – Agricultural Research Service, Robert Holly Center and Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, 14853, USA
4 Department of Plant, Soil and Entomological Sciences, University of Idaho, Moscow, ID, 83844-2339, USA
5 School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China
BMC Plant Biology 2012, 12:211 doi:10.1186/1471-2229-12-211Published: 10 November 2012
Additional file 1:
Figure S1. Phylogenetic tree of COBRA-like homologs. Phylogenetic tree generated from the alignment of SlCOBL and other plant COBRA proteins. Scale bar represents the genetic distance and node numbers indicate bootstrap support values.
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Additional file 2:
Table S1. Information about primers used in this work.
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Additional file 3:
Figure S2. Sequence alignment of tomato COBRA proteins with AtCOB and OsBC1. The alignment was generated by ClustalX . Gray and black shading indicated conservative changes and identical residues, respectively. Underlined residues corresponded to the HMM-predicted putative cellulose binding domain II. For SlCOBRA-like sequence, the Cys-rich highly conserved CCVS domain was indicated by asterisks, and conserved consensus N-glycosylation sites were indicated by black triangles.
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