Table 4

Relative CKX activity in developing and fully developed leaves and 7 DAP spikes in T1 lines of Golden Promise and one Scarlett line (S) silenced via biolistic and Agrobacterium-mediated methods
Biolistic method
Developing leaf Developed leaf Spike 7 DAP
Line* Range Mean Range Mean Range Mean
4 1.35 – 2.59 1.89 ±0.41 1.06 – 2.29 1.54 ±0.49 0.65 – 1.21 1.02 ±0.23
5** 0.78 – 4.84 2.55 ±1.57 1.01 – 14.11 3.44 ±3.83 0.40 – 1.13 0.75 ±0.28
6** 0.28 – 7.76 3.22 ±2.86 0.61 – 11.23 3.76 ±3.66 0.80 – 1.90 1.27 ±0.40
7 0.51 – 0.85 0.66 ±0.13 0.46 – 0.76 0.62 ±0.10 1.10 – 1.54 1.25 ±0.15
8 lack of germination
17 S 0.55 – 1.78 1.01 ±0.44 0.12 – 1.28 0.89 ±0.49 0.58 – 1.13 0.70 ±0.22
Agrobacterium-mediated method
Developing leaf Developed leaf Spike 7 DAP
Line Range Mean Range Mean Range Mean
411 0.64 - 1.09 0.86 ±0.19 0.90 - 1.54 1.14 ±0.26 0.51 - 0.97 0.76 ±0.19
413 0.28 - 1.04 0.74 ±0.26 0.44 - 1.14 0.80 ±0.24 0.69 - 1.12 0.93 ±0.16
415** 0.39 – 1.13 0.70 ±0.25 0.40 – 1.19 0.76 ±0.27 0.40 – 1.33 0.90 ±0.28
426 0.23 – 1.05 0.60 ±0.34 0.34 – 0.97 0.61 ±0.25 0.58 – 1.13 0.87 ±0.23
431** 0.19 – 1.08 0.66 ±0.23 0.39 – 1.54 0.88 ±0.28 0.31 – 1.44 0.90 ±0.27
443 0.35 - 0.96 0.67 ±0.22 0.42 - 1.39 0.77 ±0.36 0.49 - 1.32 0.81 ±0.33
444 0.60 - 1.21 0.90 ±0.25 0.21 - 1.08 0.56 ±0.32 0.91 - 1.18 1.03 ±0.12

* – 5 to 6 plants tested in each line.

** – 10 to 12 plants tested.

S – Scarlett line.

Lack of significant differences (P<0,05).

Zalewski et al.

Zalewski et al. BMC Plant Biology 2012 12:206   doi:10.1186/1471-2229-12-206

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