qRT-PCR analysis of mRNA isolated from pollen tubes. (A) Pollen tubes of 'Comune' and 'Monreal' grown in vitro after 24 h of culture. Scale bars: 50 μm. (B) mRNA levels of cit. 7568, cit.11563 and cit.5456 in pollen tubes grown in vitro. No expression was detected for cit.5776. Error bars indicate standard deviations from the mean. Expression levels were normalized against citrus Ubiquitin unigene contained in the GeneChip (AFFX-Cit-ubq11-3_x_at).
Caruso et al. BMC Plant Biology 2012 12:20 doi:10.1186/1471-2229-12-20