Squash preparations of self-pollinated styles with stigmas of 'Comune' and 'Monreal'. Similar behaviour of pollen germination (A, D; pictures taken 2 days after pollination) and pollen tube elongation (B, E; pictures taken 4 days after pollination) were observed in both genotypes during the early phases of pollen-pistil interaction. However, in 'Comune', pollen tubes stopped their growth in the middle style some five days after pollination (C), and no pollen tube was observed in the low style; in 'Monreal' they grew along the stylar canals (F, picture taken 6 days after pollination) and reached the base of the style 7 days after pollination. P: papillae; PT: pollen tubes; SC: stylar canals. All sections were stained by aniline blue. Scale bars: 50 μm.
Caruso et al. BMC Plant Biology 2012 12:20 doi:10.1186/1471-2229-12-20