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Open Access Research article

The BLADE-ON-PETIOLE genes of Arabidopsis are essential for resistance induced by methyl jasmonate

Juan Vicente Canet1, Albor Dobón1, Jana Fajmonová2 and Pablo Tornero3*

Author affiliations

1 Current address: Department of Crop Genetics, The John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK

2 Current address: Department of Economics and Management of Chemical and Food Industry, Faculty of Chemical Engineering, Institute of Chemical Technology Prague (ICT), Technická 5, Prague 6, Dejvice, 166 28, Czech Republic

3 Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Ciudad Politécnica de la Innovación (CPI), Ed. 8E; C/ Ingeniero Fausto Elio s/n, Valencia, 46022, Spain

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Citation and License

BMC Plant Biology 2012, 12:199  doi:10.1186/1471-2229-12-199

Published: 2 November 2012

Abstract

Background

NPR1 is a gene of Arabidopsis thaliana required for the perception of salicylic acid. This perception triggers a defense response and negatively regulates the perception of jasmonates. Surprisingly, the application of methyl jasmonate also induces resistance, and NPR1 is also suspected to be relevant. Since an allelic series of npr1 was recently described, the behavior of these alleles was tested in response to methyl jasmonate.

Results

The response to methyl jasmonate of different npr1s alleles and NPR1 paralogs null mutants was measured by the growth of a pathogen. We have also tested the subcellular localization of some npr1s, along with the protein-protein interactions that can be measured in yeast. The localization of the protein in npr1 alleles does not affect the response to methyl jasmonate. In fact, NPR1 is not required. The genes that are required in a redundant fashion are the BOPs. The BOPs are paralogs of NPR1, and they physically interact with the TGA family of transcription factors.

Conclusions

Some npr1 alleles have a phenotype in this response likely because they are affecting the interaction between BOPs and TGAs, and these two families of proteins are responsible for the resistance induced by methyl jasmonate in wild type plants.

Keywords:
Methyl jasmonate; Salicylic acid; Arabidopsis; NPR1; BOPs; Defense