Table 2

Inhibitory effect of diagnostic protease inhibitors on Botrytis cinerea secreted proteases 1
Inhibitor 2 Target proteases Working concentration (μM) Inhibitory activity (%)
Pepstatin A Asp 30 46 ± 3
PMSF Ser 3,000 15 ± 2
AEBSF Ser 300 7 ± 2
Soybean trypsin inhibitor Ser 10 7 ± 2
Leupeptin Cys/Ser 300 2 ± 2
E-64 Cys 100 3 ± 1
Chicken egg white cystatin Cys 25 3 ± 4
Oryzacystatin I Cys 25 3 ± 3
Oryzacystatin II Cys 25 2 ± 3
EDTA Metallo- 3,000 3 ± 3

1An azocaseinase assay was performed at pH 5.0, for 3 h at 37°C. Values are expressed as relative inhibitory activities compared to an uninhibited control (0% inhibition). Each datum is the mean of three values ± SE.

2AEBSF, 4-(2-aminoethyl)-benzenesulfonyl fluoride, hydrochloride; E-64, trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane; EDTA, ethylenediamine tetraacetic acid; PMSF, phenylmethylsulfonyl fluoride.

Munger et al.

Munger et al. BMC Plant Biology 2012 12:198   doi:10.1186/1471-2229-12-198

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