2-DE analysis of the leaf proteome in control line K and CCII-expressing lines 9.4 and 10.4. A. Representative 2-D gel image for the leaf proteome of line 10.4, as visualized by colloidal blue staining. A 3 to 10 pI gradient was used for isoelectric focusing. Values on the left refer to commercial molecular weight protein markers. Numbered circles refer to proteins up- or downregulated in CCII-expressing lines 9.4 and 10.4, compared to line K (see Table 1 and Additional file 1 for details). B. Relative abundance of CCII-inducible stress-related proteins in control line K and transgenic lines 9.4 and 10.4. Data are expressed as relative normalized volumes compared to line 10.4 showing the highest steady-state levels (arbitrary value of 1.0). See panel A and Table 1 for protein numbering and function assignment.
Munger et al. BMC Plant Biology 2012 12:198 doi:10.1186/1471-2229-12-198