Expression of endogenous defense proteins in leaves of control line K and CCII-expressing lines 4.1c, 9.4 and 10.4. A. mRNA transcripts for wound inducible proteinase inhibitor II (Pin-II) and pathogen inducible PR-1 protein P4, as determined by real-time RT PCR. Transcripts were assayed based on standard curves with incremental amounts of pin-II or protein p4 genes as templates. B. Immunodetection of PR-2 (β-glucanase) and PR-3 (chitinase) proteins using commercial primary antibodies directed against tobacco structural homologues. Numbers on the right refer to commercial molecular weight markers. C. Relative levels of PR-2 and PR-3 proteins in line K (relative value of 1.0) and CCII-lines, as estimated by quantitative densitometry after immunoblot digitalization. Each bar on panels A and C is the mean of three or four independent (plant replicate) values ± SE. Asterisks indicate a significant difference with control line K (Student’s t-test; P < 0.05).
Munger et al. BMC Plant Biology 2012 12:198 doi:10.1186/1471-2229-12-198