Expression of CCII and potato multicystatin in leaves of transgenic potato lines. A. CCII and ccii transcript levels in leaves of control line K and five independent transgenic lines, as determined by SELDI TOF MS and real-time RT PCR. mRNA transcripts were quantified based on a standard curve with incremental amounts of ccii transgene as a template. CCII levels are expressed as relative amounts compared to line 4.1c (mean value of 1.0). B. Immunodetection of CCII (11.7 kDa) with primary antibodies directed against oryzacystatin I, a structural homologue from rice . Numbers on the right refer to commercial molecular weight markers. PMC refers to endogenous potato multicystatin. C. Relative PMC levels in CCII-lines compared to mean basal level in control line K (arbitrary value of 1). Each bar on panels A and C is the mean of three independent (plant replicate) values ± SE. Asterisks indicate a significant difference with control line K (Student’s t-test; P < 0.05).
Munger et al. BMC Plant Biology 2012 12:198 doi:10.1186/1471-2229-12-198