|Transmission of p5cs2 mutant alleles in the presence of additional p5cs1-4 mutant alleles|
|Genotype pollen donor1||Genotype pollen recipient1||Offspring with p5cs2 allele [% ±SD2]||Expected frequency3 [%]||Seedlings analysed|
|A/a1 B/B||A/a1 B/B||65.0 ±4.0||66.7||564|
|A/a1 B/b||A/a1 B/b||52.3 ±3.4*||66.7||521|
|A/a1 b/b||A/a1 b/b||26.6 ±3.3*||66.7||545|
|A/A B/B||A/a1 b/b||43.7 ±5.1||50||332|
|A/A B/B||A/a2 b/b||50.6 ±8.2||50||212|
|A/A b/b||A/a1 b/b||44.9 ±3.4||50||382|
|A/A b/b||A/a2 b/b||44.7 ±2.4||50||232|
|A/a1 b/b||A/A B/B||0.0 ±0.0*||50||166|
|A/a2 b/b||A/A B/B||1.0 ±1.7*||50||289|
|A/a1 b/b||A/A b/b||0.0 ±0.0*||50||88|
|A/a2 b/b||A/A b/b||0.0 ± 0.0*||50||163|
Transmission of the p5cs2 mutant alleles was determined by sulfadiazine (p5cs2-1) or BASTA (p5cs2-2) resistance of the progeny after selfing (first three rows) or crossing of various parental genotypes.
1 A: P5CS2 wildtype allele; a1: p5cs2-1 mutant allele; a2: p5cs2-2 mutant allele; B: P5CS1 wildtype allele; b: p5cs1-4 mutant allele.
2 values are the mean ±SD of at least 3 independent plants or crossings.
3 the expected frequency takes into account that homozygous p5cs2/p5cs2 seeds are usually non-viable in the presence of wildtype or heterozygous seeds in the same silique (compare Additional file 2: Figure S2).
* indicates significant difference from the expected value (p<0.03 by Χ2 test with 1 degree of freedom).
Funck et al.
Funck et al. BMC Plant Biology 2012 12:191 doi:10.1186/1471-2229-12-191