Table 2

Transmission of p5cs2 mutant alleles in the presence of additional p5cs1-4 mutant alleles
Genotype pollen donor1 Genotype pollen recipient1 Offspring with p5cs2 allele [% ±SD2] Expected frequency3 [%] Seedlings analysed
A/a1 B/B A/a1 B/B 65.0 ±4.0 66.7 564
A/a1 B/b A/a1 B/b 52.3 ±3.4* 66.7 521
A/a1 b/b A/a1 b/b 26.6 ±3.3* 66.7 545
A/A B/B A/a1 b/b 43.7 ±5.1 50 332
A/A B/B A/a2 b/b 50.6 ±8.2 50 212
A/A b/b A/a1 b/b 44.9 ±3.4 50 382
A/A b/b A/a2 b/b 44.7 ±2.4 50 232
A/a1 b/b A/A B/B 0.0 ±0.0* 50 166
A/a2 b/b A/A B/B 1.0 ±1.7* 50 289
A/a1 b/b A/A b/b 0.0 ±0.0* 50 88
A/a2 b/b A/A b/b 0.0 ± 0.0* 50 163

Transmission of the p5cs2 mutant alleles was determined by sulfadiazine (p5cs2-1) or BASTA (p5cs2-2) resistance of the progeny after selfing (first three rows) or crossing of various parental genotypes.

1 A: P5CS2 wildtype allele; a1: p5cs2-1 mutant allele; a2: p5cs2-2 mutant allele; B: P5CS1 wildtype allele; b: p5cs1-4 mutant allele.

2 values are the mean ±SD of at least 3 independent plants or crossings.

3 the expected frequency takes into account that homozygous p5cs2/p5cs2 seeds are usually non-viable in the presence of wildtype or heterozygous seeds in the same silique (compare Additional file 2: Figure S2).

* indicates significant difference from the expected value (p<0.03 by Χ2 test with 1 degree of freedom).

Funck et al.

Funck et al. BMC Plant Biology 2012 12:191   doi:10.1186/1471-2229-12-191

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