Table 1 |
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| Transmission of p5cs2 mutant alleles by self-pollination and in reciprocal crosses with wildtype plants | |||||
| Genotype pollen donor1 | Genotype pollen recipient1 | Treatment | Offspring with p5cs2 allele [% ±SD2] | Expected frequency3 [%] | Seedlings analysed |
| A/a1 | A/a1 | none | 58.9 ±1.3* | 66.7 | 474 |
| A/a1 | A/a1 | 50 mM NaCl | 64.1 ±0.5 | 66.7 | 457 |
| A/a2 | A/a2 | none | 59.1* | 66.7 | 164 |
| A/a2 | A/a2 | 50 mM NaCl | 68.2 | 66.7 | 176 |
| A/A | A/a1 | none | 46.4 | 50 | 489 |
| A/A | A/a2 | none | 40.8* | 50 | 309 |
| A/a1 | A/A | none | 46.1 | 50 | 371 |
| A/a2 | A/A | none | 47.7 | 50 | 461 |
Transmission of the p5cs2 mutant alleles was determined by sulfadiazine (p5cs2-1) or BASTA (p5cs2-2) resistance of the progeny after selfing (first four rows) or reciprocal crossing of heterozygous p5cs2 mutants with wildtype plants.
1 A: P5CS2 wildtype allele; a1: p5cs2-1 mutant allele; a2: p5cs2-2 mutant allele.
2 SD was calculated from three batches of seeds from individual parent plants.
3 The expected frequency takes into account that homozygous p5cs2/p5cs2 seeds are usually non-viable in the presence of wildtype or heterozygous seeds in the same silique (compare Additional file 2 FigureS2).
* indicates significant difference from the expected value (p<0.05 by Χ2 test with 1 degree of freedom).
Funck et al. BMC Plant Biology 2012 12:191 doi:10.1186/1471-2229-12-191
