|Transmission of p5cs2 mutant alleles by self-pollination and in reciprocal crosses with wildtype plants|
|Genotype pollen donor1||Genotype pollen recipient1||Treatment||Offspring with p5cs2 allele [% ±SD2]||Expected frequency3 [%]||Seedlings analysed|
|A/a1||A/a1||50 mM NaCl||64.1 ±0.5||66.7||457|
|A/a2||A/a2||50 mM NaCl||68.2||66.7||176|
Transmission of the p5cs2 mutant alleles was determined by sulfadiazine (p5cs2-1) or BASTA (p5cs2-2) resistance of the progeny after selfing (first four rows) or reciprocal crossing of heterozygous p5cs2 mutants with wildtype plants.
1 A: P5CS2 wildtype allele; a1: p5cs2-1 mutant allele; a2: p5cs2-2 mutant allele.
2 SD was calculated from three batches of seeds from individual parent plants.
3 The expected frequency takes into account that homozygous p5cs2/p5cs2 seeds are usually non-viable in the presence of wildtype or heterozygous seeds in the same silique (compare Additional file 2 FigureS2).
* indicates significant difference from the expected value (p<0.05 by Χ2 test with 1 degree of freedom).
Funck et al. BMC Plant Biology 2012 12:191 doi:10.1186/1471-2229-12-191