ROS detection in SaSce9 transgenic Arabidopsis plants. The leaves of unstressed and 7 days drought stressed WT and the SaSce9 transgenic Arabidopsis plants were immersed in 1 mg/ml fresh NBT solution, (a) Photograph was taken after washing with ethanol, and (b) Quantification of stained spots were done using Adobe Photoshop. Experiment was repeated at least three times using rosette leaves from each WT and 35S-SaSce9 plants and photograph represents one of the three experiments. Vertical bar represents mean and error bar represent standard error of means based on three independent experiments.
Karan and Subudhi BMC Plant Biology 2012 12:187 doi:10.1186/1471-2229-12-187