RT-PCR and qRT-PCR on laser microdissected cells. (A) RT-PCR analysis of LjNIP1 and LjXIP1 in LMD samples: LjNIP1 amplified fragments were only detected in arbusculated cells (ARB), whereas LjXIP1 fragments were also detected in non-colonized cortical cells from mycorrhizal roots (MNM) and in cortical cells from non-mycorrhizal roots (C). (B) Relative expression of LjXIP1 in LMD samples assessed by means of One-Step qRT-PCR. The highest level was detected in the arbusculated cells. The values are the means of two biological samples and two technical replicates, with bars representing the standard deviations.
Giovannetti et al. BMC Plant Biology 2012 12:186 doi:10.1186/1471-2229-12-186