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Open Access Methodology article

Quantitative RT-PCR based platform for rapid quantification of the transcripts of highly homologous multigene families and their members during grain development

Agnieszka Kaczmarczyk1, Steve Bowra2, Zoltan Elek3 and Eva Vincze1*

Author Affiliations

1 Department of Genetics and Biotechnology, Aarhus University, Research Centre Flakkebjerg, Forsøgsvej 1, Slagelse, DK-4200, Denmark

2 Verzyme (UK) Ltd., Plas Gogerddan, Aberystwyth, Wales, SY23 3EB, United Kingdom

3 MTA-ELTE-MTM Ecology Research Group, Biological Institute, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, H-1117, Hungary

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BMC Plant Biology 2012, 12:184  doi:10.1186/1471-2229-12-184

Published: 9 October 2012

Additional files

Additional file 1:

Alignment of B-, C-, D- and γ-hordein sequences found in NCBI databases. A location of qRT-PCR primers is highlighted.

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Open Data

Additional file 2:

Abundance of hordein groups during grain development using actin for normalisation.

Format: PDF Size: 38KB Download file

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Open Data