Figure 4.

FIL-response genes that areimmediate targets of FIL. (A) Fold change in expression of FIL-response genes in ten-day-old 35S_pro::FIL:GR seedlings following a 4 h DEX or DEX/CHX treatment. Brackets indicate genes that display significant transcriptional responses to both treatments and hence mark direct targets of FIL. (B) Response of selected positively regulated FIL-response genes in 10 day-old FIL_pro::FIL:GR seedlings following a 4 h DEX treatment. (C,D) Induction of abaxial polarity regulators in 10 day-old 35S_pro::FIL:GR (C) or 35S_pro::YAB3:GR (D) seedlings following a 4 h DEX treatment. (E) Expression of KAN1, ARF4 and AS1 in fil single and yab double, triple and quadruple mutants. Expression in a minimum of three biological replicates was determined using quantitative real-time RT-PCR and normalized first to a housekeeping gene and then to mock treatment controls. Asterisks mark significant differences determined by a Student’s t-test (one asterisk, 0.01<p<0.05; two asterisks, 0.001<p<0.005) and error bars are SEM. The grey line marks the expression level expected if there is no response to treatment.