FILAMENTOUS FLOWER controls lateral organ development by acting as both an activator and a repressor
- Equal contributors
1 Department of Genetics, University of Melbourne, Royal Parade, Parkville, VIC 3010, Australia
2 Laboratoire de Reproduction et Développement des Plantes, UMR 5667- CNRS/INRA/Université de Lyon, École Normale Supérieure de Lyon, 46, allée d'Italie 69364, Lyon Cedex, 07, France
BMC Plant Biology 2012, 12:176 doi:10.1186/1471-2229-12-176Published: 1 October 2012
Additional file 1:
Microarray analysis of 35Spro::FIL:GR seedlings following dexamethasone treatment. This file contains lists of genes that were identified as being differentially expressed in the shoot tissue of 35Spro::FIL:GR lines exposed to DEX for 4 h or 8 h.
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Additional file 2:
FIL-response genes that are differentially expressed at 4 h and 8 h following DEX treatment or at 8 h following DEX treatment. This file contains lists of genes that are have been placed into two groups. Group A genes are differentially expressed at 4 h and 8 h following DEX treatment, whereas Group B genes are differentially expressed at 8 h but not 4 h following DEX treatment. (
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Additional file 3:
Histochemical staining of seedlings treated with DEX and CHX. This figure shows the effectiveness of DEX and DEX/CHX treatments on control plants. Histochemical staining for GUS activity in ten-day-old 35SproI> > GUS seedlings exposed to a mock DEX/CHX treatment (A), CHX (B), DEX (C) and DEX/CHX (D) for 9 h. Scale bars are 1 mm.
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Additional file 4:
Vegetative phenotypes associated with steroid-induced activation of abaxially expressed FILand constitutively expressed YAB3. This figure shows the phenotype of FILpro::FIL:GR plants and 35Spro::YAB3:GR plants continuously exposed to DEX. (A) Fourteen-day-old FILpro::FIL:GR plants grown on soil and sprayed with DEX (see Methods). (B,C) 35Spro::YAB3:GR plant grown on media without DEX (B) or in the presence of DEX (C). Scale bars are 1 mm.
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Additional file 5:
Vegetative phenotype associated with continuous FIL activation in different mutants backgrounds. This figure shows the DEX-inducible phenotype and leaf epidermal cell morphology of mutant plants harbouring the 35Spro::FIL:GR construct. (A-D) Twenty-day-old 35Spro::FIL:GR (A), 35Spro::FIL:GR/arf4 (B), 35Spro::FIL:GR/kan1 (C) and 35Spro::FIL:GR/arf4 kan1 (D), plants grown on soil and sprayed with DEX (see Methods). (E-I) SEM of the adaxial surface of a leaf taken from 35Spro::FIL:GR plants (E, F), a 35Spro::FIL:GR/arf4 plant (G), a 35Spro::FIL:GR/kan1 plant (H) or a 35Spro::FIL:GR/arf4 kan1 plant (I). Plants received a mock treatment (E) or were sprayed with DEX (F-I). Scale bars are 2 mm (A-D) and 100 μM (E-I).
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Additional file 6:
Primer sequences used for qRT-PCR and cloning. This file contains a list of all the oligonucleotides used for generating constructs and conducting qRT-PCR.
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