Open Access Highly Accessed Research article

High-throughput sequencing of black pepper root transcriptome

Sheila MC Gordo1, Daniel G Pinheiro2, Edith CO Moreira1, Simone M Rodrigues3, Marli C Poltronieri3, Oriel F de Lemos3, Israel Tojal da Silva2, Rommel TJ Ramos4, Artur Silva4, Horacio Schneider1, Wilson A Silva2, Iracilda Sampaio1 and Sylvain Darnet4*

Author Affiliations

1 Genetics and Molecular Biology Laboratory, Coastal Studies Institute, Bragança Campus, Universidade Federal do Pará, Bragança, PA, 68.600-000, Brazil

2 Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Centro Regional de Hemoterapia de Ribeirão Preto, Rua Tenente Catão Roxo, 2501, Ribeirão Preto, SP 14051-140, Brazil

3 EMBRAPA Amazônia Oriental, Trav. Dr. Enéas Pinheiro s/nº, Caixa Postal 48, Belém, PA 66095-100, Brazil

4 Instituto de Ciências Biológicas, Universidade Federal do Pará, Campus Universitário do Guamá, Rua Augusto Corrêa, nº1, Belém, PA 66075-110, Brazil

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BMC Plant Biology 2012, 12:168  doi:10.1186/1471-2229-12-168

Published: 17 September 2012

Additional files

Additional file 1 :

Figure S1. Statistical analysis of quality value (QV) of sequencing dataset. The quartiles, median and average were plotted for each position of the dataset of reads.

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Additional file 2 :

Figure S2. Size distribution of the filtered and trimmed reads from 2-GS sequencing from the black pepper root.

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Additional file 3 :

Figure S3. Picture of black pepper root used for cDNA library. A) Global view of 70 day old plantlets. The red box indicates the root region used to extract RNAs. B) Sectional view of black pepper root at the region used to extract RNAs.

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