Table 1

Transcripts putatively involved in the secondary metabolism of olive fruits
Pathway Transcript Accession Number Length (bp) Enzymatic functionb Blast resultsc
MEP pathway OeDXS* JX266162 574 1-deoxy-d-xylulose 5-phosphate synthase (EC: 7.9E-124, 92% (H. brasiliensis)
OeDXR JX266164 596 1-deoxy-d-xylulose-5-phosphate reductoisomerase (EC: 2.5E-129, 92% (H. brasiliensis)
OeCDPMES JX266166 927 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (EC: 4.0E-141, 87% (S. miltiorrhiza)
OeCDPMEK JX266168 800 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC: 5.1E-111, 76% (S. miltiorrhiza)
OeMECPS JX266170 550 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC: 5.8E-89, 75% (A. adenophora)
OeHMBPPS JZ030838 178 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase-like (EC 9.0E-32, 95% (V. vinifera)
OeHMBPPR* JX266172 1114 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC: 0.0, 87% (S. lycopersicum)
OeIPPI JX266174 840 Isopentenyl diphosphate isomerase (EC: 2.3E-156, 95% (N. tabacum)
Mevalonate pathway (MVA) OeHMGR JZ030840 214 3-hydroxy-3-methyl glutaryl CoA reductase (EC: 7.6E-40, 89% (S. nigrum)
OeMVAK JX266176 1152 Mevalonate kinase (EC: 4.1E-142, 84% (C. roseus)
OeMVAPK JX266178 366 Phosphomevalonate kinase (EC: 6.2E-24, 67% (V. vinifera)
OeMVAPPD JX266179 373 Mevalonate diphosphate decarboxylase (EC: 1.0E-14, 85% (C. roseus)
Synthesis of monoterpenic moiety of secoiridoids OeGES JX266180 1090 geraniol synthase (EC:4.2.3.-) 4.0E-166, 76% (P.dulcis)
OeGE10H JX266182 1232 Geraniol 10-hydroxylase (EC:1.14.13.B15) 0.0, 80% (C. roseus)
OeNDHD* GQ851611 643 NADH dehydrogenase I (EC: 1.0E-92, 77% (P. trichocarpa x P. deltoides)
OeGT* GQ851612 1050 Glucosyltransferase (EC:2.4.1) 8.0E-126, 69% (N. tabacum)
OeSLS1 JX266184 907 Secologanin synthase-like (EC: 1.2E-145, 67% (V. vinifera)
OeSLS2 JX266186 1325 Secologanin synthase-like (EC: 1.1E-120, 45% (G. max)
OeSLS3 JX266188 832 Secologanin synthase-like (EC: 1.8E-79, 44% (G. max)
OeSLS4 JX266190 667 Secologanin synthase (EC: 1.3E-44, 38% (C. roseus)
OeLAMT JX266191 572 S-adenosylmethionine-dependent methyltransferase (EC: 1.9E-65, 53% (V. vinifera)
Synthesis of phenolic moieties OeADH* GQ851610 790 Arogenate dehydrogenase (EC: 6.0E-70, 84% (S. pennellii)
OeCuAO* GQ851613 1843 Copper amine oxidase (EC: 0.0, 89% (R. communis)
OePPO JX266193 1491 Polyphenol oxidase (EC: 0.0, 60% (S. indicum)
OeTYRD JX266195 1373 Tyrosine/dopa decarboxylase (EC: 0.0, 78% (P. somniferum)
OeALDH1 JX266197 793 Alcohol dehydrogenase (EC: 2.0E-163, 98% (O. europaea)
OeALDH2 JX266199 487 Alcohol dehydrogenase class-3 (EC: 1.0E-95, 93% (G.max)
Phenylpropanoid biosynthesis OePAL JX266200 1587 Phenylalanine ammonia-lyase (EC: 0.0, 90% (P. frutescens)
Oe4CL JX266202 260 4-coumarate coenzyme A ligase (EC: 9.1E-50, 98% (P. fortunei)
Sterol and terpene biosynthesis OeLS JZ030839 169 Limonene synthase like (EC: 6.0E-17, 67% (A. thaliana)
OeFPPS JX266204 1011 Farnesyl diphosphate synthase (EC: 0.0, 89% (G. uralensis)
OeSQS JX266206 516 Squalene synthase (EC: 1.2E-111, 95% (B. monnieri)
OeGGPS JX266207 752 Geranylgeranyl pyrophosphate synthase (EC: 2.1E-159, 90% (C. roseus)
OeLUPSd AB025343d
Degradation of phenolics OeGLU* HQ585436 857 Beta-1,3-glucosidase (EC: 6.0E-85, 85% (R. communis)
OePOX* GQ851609 1182 Peroxidase (EC: 4.0E-140, 82% (R. communis)

a The GenBank accession numbers of the transcripts are provided. Numbers JZ030838, JZ030840 and JZ030839 refer to the dbEST collection.

b Putative enzymatic function and Enzyme Commission (EC) number are provided.

c The E-value and the percentage identity from the best hit of the BLASTX search are provided. These values were used to indicate the significance of sequence similarity. The text in parentheses indicates the species of the best hit from BLASTX search.

d Transcript sequenced and characterised by Shibuya et al.[28].

* Transcripts derived from the cDNA-AFLP and RACE PCR analyses.

Alagna et al.

Alagna et al. BMC Plant Biology 2012 12:162   doi:10.1186/1471-2229-12-162

Open Data