Susceptibility to Dickeya chrysanthemi (D. chrysanthemi) and Fusarium verticillioides (F.verticillioides ) of control untransformed Senia and S-bp100.1, S-bp100.2i, S-bp100.2mi and S-hgr GM plants in a germination assay.(A) Representative examples of seedlings with different semi-quantitative development values to estimate D.chrysanthemi effects: 0, no germination; 0.5, cotyledon development; 1, standard shoot but no root development; 1.5, standard shoot development but short roots; and 2, standard growth. (B) Means and standard errors of seedling development values are represented as a function of the transgene in each line (every homozygous line obtained in this work was analyzed). Both for D. chrysanthemi and F. verticillioides susceptibility, a value of 0 indicated no germination while a value of 2 represented development similar to uninfected seeds. Asterisks indicate statistically significant differences compared to Senia (one-way ANOVA P values are given in the text). (C) Representative examples of S-bp100.2i (left) and Senia (center) seedlings grown in the presence of 105 CFU D. chrysanthemi. Germination of Senia control seeds (right). (D) Representative examples of S-bp100.2mi (left) and Senia (center) seedlings grown in the presence of 105CFU F. verticillioides and germination of Senia control seeds (right). Scale bars: 1 cm.
Nadal et al. BMC Plant Biology 2012 12:159 doi:10.1186/1471-2229-12-159