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Open Access Highly Accessed Research article

Identification and characterization of small non-coding RNAs from Chinese fir by high throughput sequencing

Li-Chuan Wan1, Feng Wang12, Xiangqian Guo3, Shanfa Lu4, Zongbo Qiu1, Yuanyuan Zhao12, Haiyan Zhang1* and Jinxing Lin1*

Author Affiliations

1 Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

2 Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China

3 Bioinformatics Laboratory and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

4 Medicinal Plant Cultivation Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haidian District, Beijing, 100193, China

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BMC Plant Biology 2012, 12:146  doi:10.1186/1471-2229-12-146

Published: 15 August 2012

Additional files

Additional file 1:

Common sRNAs matching perfectly to the Chinese fir mRNA transcriptome,A. thalianaandP. trichocarpagenomes.

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Open Data

Additional file 2:

Conserved and novel miRNAs and tasiRNAs in Chinese fir.

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Additional file 3:

Precursor and primary sequences of conserved and novel miRNAs in Chinese fir.

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Additional file 4:

The hairpin structures of conserved and novel miRNAs predicted by MFOLD.

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Additional file 5:

RT-PCR of conserved mature miRNAs.

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Additional file 6:

Unigenes involved in the biogenesis and action of miRNAs in Chinese fir.

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Additional file 7:

Conserved and novel miRNA targets and their putative functions.

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Additional file 8:

Dicer-like proteins in Chinese fir.

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Additional file 9:

Phylogenetic trees of unigene18579 and unigene16720 with three model plant DCLs.

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Additional file 10:

Unigenes involved in the biogenesis and action of 24-nt rasiRNAs in Chinese fir.

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Additional file 11:

Unigenes involved in the biogenesis and action of tasiRNAs in Chinese fir.

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Additional file 12:

Total unigenes in Chinese fir.

Format: ZIP Size: 9.1MB Download file

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Additional file 13:

Primers for RT-PCR, qRT-PCR and 5' RACE.

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