Expression of defence genes on G. cichoracearum-infected tissues. (a) Northern blots showing a time-course expression experiment for genes sensitive to ROS (GST) or SA accumulation (PR1) on Col-0 (C), Te-0 (T), and Kas-1 (K) plants. RNA was extracted from leaves at the indicated times post-infection with G. cichoracearum. (b) Semi-quantitative RT-PCR assays were used to evaluate the expression of the JA-sensitive genes VSP2, LOX2 and PDF1.2 on samples similar to those described on (a). GapC amplification was included as control for cDNA input. (c) Expression pattern of the GH14 and TURP genes sensitive to the SA and JA pathways determined by semi-quantitative RT-PCR. These results were verified by qRT-PCR as shown in Additional file 2.
Fabro and Alvarez BMC Plant Biology 2012 12:143 doi:10.1186/1471-2229-12-143