Additional file 1.

Figure S1: Flow-chart summarizing the main steps of the NGS-BSA approach. Figure S2: Chromatograms of LDN and RSL65 for Ta#S32574498. Figure S3: BFR of validated and mapped SNPs across the GPC-B1 interval. Figure S4: Mapping of wheat unigenes with putative SNP to the Brachypodium genome. Figure S5: View of MAQ alignment of Ta#S37941845 reads for LDN and RSL65 encompassing the hemi-SNP (R = A/G) at position 582. Figure S6: Visualization of fluorescence output for two hemi-SNPs Table S1: Breakdown of SNPs lost during the validation and mapping process Table S2: Collinearity between most closely identified markers in the present study and previous markers used for physical map construction.

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Trick et al. BMC Plant Biology 2012 12:14   doi:10.1186/1471-2229-12-14